- Abstract:
-
The performance of a method is assessed which allows for the spatiotemporal tracking of single dye-labeled molecules during two-dimensional (2D) diffusional transits through the focal area of a modified confocal microscope. In addition to facilitating the observation of molecular diffusion paths at the shot-noise limit of bright organic emitters with spatial and temporal precisions of ∼10-20 nm and <0.5 ms, respectively, the direct access to the complete stream of detected photons is benef...
Expand abstract - Publisher copy:
- 10.1002/cphc.201301090
-
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- Publisher:
- Wiley-VCH Verlag
- Journal:
- ChemPhysChem
- Volume:
- 15
- Issue:
- 4
- Pages:
- 771-783
- Publication date:
- 2014-03-17
- DOI:
- EISSN:
-
1439-7641
- ISSN:
-
1439-4235
- URN:
-
uuid:33134127-61d5-46b5-85a8-3a9427743c3f
- Source identifiers:
-
460198
- Local pid:
- pubs:460198
- Language:
- English
- Keywords:
- Copyright date:
- 2014
Journal article
High-resolution tracking of single-molecule diffusion in membranes by confocalized and spatially differentiated fluorescence photon stream recording
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