Journal article icon

Journal article

High-resolution tracking of single-molecule diffusion in membranes by confocalized and spatially differentiated fluorescence photon stream recording

Abstract:

The performance of a method is assessed which allows for the spatiotemporal tracking of single dye-labeled molecules during two-dimensional (2D) diffusional transits through the focal area of a modified confocal microscope. In addition to facilitating the observation of molecular diffusion paths at the shot-noise limit of bright organic emitters with spatial and temporal precisions of ∼10-20 nm and <0.5 ms, respectively, the direct access to the complete stream of detected photons is benef...

Expand abstract

Actions


Access Document


Publisher copy:
10.1002/cphc.201301090

Authors


Publisher:
Wiley-VCH Verlag
Journal:
ChemPhysChem
Volume:
15
Issue:
4
Pages:
771-783
Publication date:
2014-03-17
DOI:
EISSN:
1439-7641
ISSN:
1439-4235
URN:
uuid:33134127-61d5-46b5-85a8-3a9427743c3f
Source identifiers:
460198
Local pid:
pubs:460198

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP