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Influence of segmenting fluids on efficiency, crossing point and fluorescence level in real time quantitative PCR.

Abstract:

The two-phase segmented flow approach to the processing and quantitative analysis of biological samples in microdevices offers significant advantages over the single-phase continuous flow methodology. Despite this, little is known about the compatibility of samples and reactants with segmenting fluids, although a number of investigators have reported reduced yield and inhibition of enzymatic reactions depending on the segmenting fluid employed. The current study addresses the compatibility of...

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Publisher copy:
10.1007/s10544-006-6383-9

Authors


Gonzalez, A More by this author
Journal:
Biomedical microdevices
Volume:
8
Issue:
1
Pages:
59-64
Publication date:
2006-03-05
DOI:
EISSN:
1572-8781
ISSN:
1387-2176
URN:
uuid:31fd045e-0760-4ec4-aabb-0843553cdcbc
Source identifiers:
369430
Local pid:
pubs:369430

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