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Mammalian NET-seq analysis defines nascent RNA profiles and associated RNA processing genome-wide

Abstract:

The transcription cycle of RNA polymerase II (Pol II) correlates with changes to the phosphorylation state of its large subunit C-terminal domain (CTD). We recently developed Native Elongation Transcript sequencing using mammalian cells (mNET-seq), which generates single-nucleotide–resolution genome-wide profiles of nascent RNA and co-transcriptional RNA processing that are associated with different CTD phosphorylation states. Here we provide a detailed protocol for mNET-seq. First, Pol II el...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/nprot.2016.012

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Role:
Author
ORCID:
0000-0003-1333-0191
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
Oxford college:
Lincoln College
Role:
Author
Publisher:
Springer Nature Publisher's website
Journal:
Nature Protocols Journal website
Volume:
11
Issue:
03
Pages:
413–428
Publication date:
2016-02-04
Acceptance date:
2015-02-25
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Pubs id:
pubs:601495
URN:
uri:31b1c9e5-61ea-4f55-b488-b1214b9af006
UUID:
uuid:31b1c9e5-61ea-4f55-b488-b1214b9af006
Local pid:
pubs:601495
Language:
English
Keywords:

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