Journal article
Mammalian NET-seq analysis defines nascent RNA profiles and associated RNA processing genome-wide
- Abstract:
-
The transcription cycle of RNA polymerase II (Pol II) correlates with changes to the phosphorylation state of its large subunit C-terminal domain (CTD). We recently developed Native Elongation Transcript sequencing using mammalian cells (mNET-seq), which generates single-nucleotide–resolution genome-wide profiles of nascent RNA and co-transcriptional RNA processing that are associated with different CTD phosphorylation states. Here we provide a detailed protocol for mNET-seq. First, Pol II el...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Publisher copy:
- 10.1038/nprot.2016.012
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Authors
Funding
Bibliographic Details
- Publisher:
- Springer Nature Publisher's website
- Journal:
- Nature Protocols Journal website
- Volume:
- 11
- Issue:
- 03
- Pages:
- 413–428
- Publication date:
- 2016-02-04
- Acceptance date:
- 2015-02-25
- DOI:
- EISSN:
-
1750-2799
- ISSN:
-
1754-2189
- Pmid:
-
26844429
- Source identifiers:
-
601495
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:601495
- UUID:
-
uuid:31b1c9e5-61ea-4f55-b488-b1214b9af006
- Local pid:
- pubs:601495
- Deposit date:
- 2019-06-10
Terms of use
- Copyright holder:
- Springer Nature
- Copyright date:
- 2016
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