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Foundations of STED microscopy

Abstract:

This chapter presents the foundations of STED microscopy with a comparison to its generalization Resolft microscopy and to stochastic imaging methods (PALM, STORM, FPALM, and alike). The first section reviews the advantages of optical microscopy, explains the diffraction limit, and shows how the classical resolution limit was finally broken. It also reviews some of the achievements in super-resolution imaging. The second section explains in depth the principle of STED microscopy and highlight...

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Publisher copy:
10.1007/978-1-62703-983-3-3

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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
Role:
Author
Publisher:
Humana Press Inc.
Journal:
Neuromethods
Volume:
86
Pages:
41-71
Publication date:
2014-01-01
DOI:
EISSN:
1940-6045
ISSN:
0893-2336
URN:
uuid:316a1307-3d55-4a20-9e80-6adbfc7b4f45
Source identifiers:
460199
Local pid:
pubs:460199

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