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Effects of insulin-free plasma on the charcoal-separation method for radioimmunoassay of insulin.

Abstract:

Radioimmunoassay of insulin in rat plasma using a popular method (Albano, Ekins, Maritz and Turner 1972) involving charcoal-separation of free and antibody-bound insulin was found to be unsatisfactory despite inclusion in standard tubes of insulin-free plasma prepared in either of two ways. Insulin-free plasma and untreated plasma had different effects on adsorption of free insulin to the charcoal. It was concluded that separation with charcoal is very sensitive to any prior treatment of the ...

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Publication status:
Published

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Publisher copy:
10.1055/s-0028-1093681

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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, OCDEM
Journal:
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme
Volume:
8
Issue:
2
Pages:
102-105
Publication date:
1976-03-05
DOI:
EISSN:
1439-4286
ISSN:
0018-5043
URN:
uuid:315f6daa-4c24-492d-beda-4815d1881527
Source identifiers:
20677
Local pid:
pubs:20677

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