Charge-state dependent compaction and dissociation of protein complexes: insights from ion mobility and molecular dynamics.

Journal article

Collapse to compact states in the gas phase, with smaller collision cross sections than calculated for their native-like structure, has been reported previously for some protein complexes although not rationalized. Here we combine experimental and theoretical studies to investigate the gas-phase structures of four multimeric protein complexes during collisional activation. Importantly, using ion mobility-mass spectrometry (IM-MS), we find that all four macromolecular complexes retain their na...

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Authors: Hall, Z; Politis, A; Bush, M; Smith, L; Robinson, C

• Publication status: Published
• Journal: Journal of the American Chemical Society
• Volume: 134
• Issue: 7
• Pages: 3429-3438
• Publication date: 2012-02-01
• DOI: 10.1021/ja2096859
• EISSN: 1520-5126
• ISSN: 0002-7863
• Language: English
• Keywords: Multiprotein Complexes; Protein Unfolding; Bacterial Proteins; Animals; Molecular Dynamics Simulation; Ions; Humans; Bacillus subtilis