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Journal article

High-throughput class I HLA genotyping using fluorescence resonance energy transfer (FRET) probes and sequence-specific primer-polymerase chain reaction (SSP-PCR).

Abstract:

We have developed a semi-automated HLA class I typing system utilising TET/TAMRA-labelled fluorescence resonance energy transfer (FRET) hydrolysis probes. The results from 87 individuals are in full concordance with serology and conventional gel-based systems. This assay replaces labour-intensive conventional gel-based DNA typing and has a higher allelic resolution than serology. Our approach differs from previously published fluorogenic probe typing protocols in that it provides simultaneous...

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Publication status:
Published

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Authors


Gelsthorpe, AR More by this author
Bodmer, JG More by this author
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Journal:
Tissue antigens
Volume:
54
Issue:
6
Pages:
603-614
Publication date:
1999-12-05
DOI:
EISSN:
1399-0039
ISSN:
0001-2815
URN:
uuid:307ade23-0ac1-43d3-81e1-e178f94e83ff
Source identifiers:
487571
Local pid:
pubs:487571

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