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A novel membrane based process to isolate photosystem-I membrane complex from spinach.

Abstract:

The isolation of photosystem-I (PS-I) from spinach has been conducted using ultrafiltration with 300 kDa molecular weight cut-off polyethersulfone membranes. The effects of ultrafiltration operating conditions on PS-I activity were optimized using parameter scanning ultrafiltration. These conditions included solution pH, ionic strength, stirring speed, and permeate flux. The effects of detergent (Triton X-100 and n-dodecyl-beta-D-maltoside) concentration on time dependent activity of PS-I wer...

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Publication status:
Published

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Publisher copy:
10.1007/s11120-011-9625-5

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Journal:
Photosynthesis research
Volume:
107
Issue:
2
Pages:
187-193
Publication date:
2011-02-05
DOI:
EISSN:
1573-5079
ISSN:
0166-8595
URN:
uuid:3054dae2-017b-4533-8915-512974dd6901
Source identifiers:
124470
Local pid:
pubs:124470

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