The dynamic localisation of the Drosophila APC/C: evidence for the existence of multiple complexes that perform distinct functions and are differentially localised.

Journal article

In Drosophila cells, the destruction of cyclin B is spatially regulated. In cellularised embryos, cyclin B is initially degraded on the mitotic spindle and is then degraded in the cytoplasm. In syncytial embryos, only the spindle-associated cyclin B is degraded at the end of mitosis. The anaphase promoting complex/cyclosome (APC/C) targets cyclin B for destruction, but its subcellular localisation remains controversial. We constructed GFP fusions of two core APC/C subunits, Cdc16 and Cdc27. T...

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Authors: Huang, J; Raff, J

• Publication status: Published
• Journal: Journal of cell science
• Volume: 115
• Issue: Pt 14
• Pages: 2847-2856
• Publication date: 2002-07-01
• EISSN: 1477-9137
• ISSN: 0021-9533
• Language: English
• Keywords: RNA Interference; Drosophila melanogaster; Ubiquitin-Protein Ligase Complexes; Recombinant Fusion Proteins; Mutation; Luminescent Proteins; Ligases; RNA, Small Interfering; Cyclin A; Embryo, Nonmammalian; RNA, Double-Stranded; Mitosis; Animals; Green Fluorescent Proteins; Cell Cycle Proteins; Drosophila Proteins; Cell Compartmentation; Cyclin B