Identification of the mouse beta'-COP Golgi component as a spermatocyte autoantigen in scleroderma and mapping of its gene Copb2 to mouse chromosome 9.

Journal article

In an immunological screening of a mouse testicular cDNA library with a human CREST serum we isolated five overlapping cDNA clones encoding the mouse homolog of a Golgi coatomer complex protein (accession number AF043120), designated beta'-COP in bovine and p102 in humans. We generated antibodies against this protein which specifically recognize the Golgi apparatus of mouse spermatocytes. FISH analyses assigned the beta'-COP gene Copb2 to mouse Chromosome 9, region E3-F1. Our results demonstrate that CREST sera can contain antibody components against Golgi proteins as well as against nuclear proteins.

Authors: Tarsounas, M; Heng, H; Ye, C; Pearlman, R; Moens, P

• Publication status: Published
• Journal: Cytogenetics and cell genetics
• Volume: 87
• Issue: 3-4
• Pages: 201-204
• Publication date: 1999-01-01
• DOI: 10.1159/000015467
• EISSN: 1424-859X
• ISSN: 0301-0171
• Language: English
• Keywords: Humans; In Situ Hybridization, Fluorescence; Animals; Autoantigens; Immune Sera; Male; Blotting, Western; Molecular Sequence Data; Antibody Specificity; Sequence Homology, Amino Acid; Mice; Golgi Apparatus; Physical Chromosome Mapping; Spermatocytes; Cloning, Molecular; Coatomer Protein; Gene Library; CREST Syndrome