A high throughput perfusion-based microbioreactor platform integrated with pneumatic micropumps for three-dimensional cell culture.

Journal article

This study reports a new perfusion-based, micro three-dimensional (3-D) cell culture platform for high-throughput cell culture using enabling microfluidic technologies. In this work, the micro 3-D cell culture platform is fabricated based on SU-8 lithography and polydimethylsiloxane replication processes. The micro cell culture platform can maintain homogenous and stable culture environments, as well as provide pumping of multiple mediums and efficient cell/agarose (scaffold) loading function...

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Authors: Wu, M; Huang, S; Cui, Z; Cui, Z; Lee, G

• Publication status: Published
• Journal: Biomedical microdevices
• Volume: 10
• Issue: 2
• Pages: 309-319
• Publication date: 2008-04-01
• DOI: 10.1007/s10544-007-9138-3
• EISSN: 1572-8781
• ISSN: 1387-2176
• Language: English
• Keywords: Microfluidic Analytical Techniques; Bioreactors; Equipment Design; Perfusion; Flow Injection Analysis; Systems Integration; Cell Culture Techniques; Equipment Failure Analysis