- Abstract:
-
We report a substantial signal gain in fluorescence microscopy by ensuring that transient molecular dark states with lifetimes >1 micros, such as the triplet state relax between two molecular absorption events. For GFP and Rhodamine dye Atto532, we observed a 5-25-fold increase in total fluorescence yield before molecular bleaching when strong continuous-wave or high-repetition-rate pulsed illumination was replaced with pulses featuring temporal pulse separation >1 micros. The signal ga...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1038/nmeth986
-
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- Journal:
- Nature methods
- Volume:
- 4
- Issue:
- 1
- Pages:
- 81-86
- Publication date:
- 2007-01-05
- DOI:
- EISSN:
-
1548-7105
- ISSN:
-
1548-7091
- URN:
-
uuid:2e0ea7cb-1a18-4fe5-81a7-811b0aae0d30
- Source identifiers:
-
222170
- Local pid:
- pubs:222170
- Language:
- English
- Keywords:
- Copyright date:
- 2007
Journal article
Major signal increase in fluorescence microscopy through dark-state relaxation.
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