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Major signal increase in fluorescence microscopy through dark-state relaxation.

Abstract:

We report a substantial signal gain in fluorescence microscopy by ensuring that transient molecular dark states with lifetimes >1 micros, such as the triplet state relax between two molecular absorption events. For GFP and Rhodamine dye Atto532, we observed a 5-25-fold increase in total fluorescence yield before molecular bleaching when strong continuous-wave or high-repetition-rate pulsed illumination was replaced with pulses featuring temporal pulse separation >1 micros. The signal ga...

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Publication status:
Published

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Publisher copy:
10.1038/nmeth986

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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
Role:
Author
Journal:
Nature methods
Volume:
4
Issue:
1
Pages:
81-86
Publication date:
2007-01-05
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
URN:
uuid:2e0ea7cb-1a18-4fe5-81a7-811b0aae0d30
Source identifiers:
222170
Local pid:
pubs:222170

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