- Abstract:
-
An efficient and easily scaled up method to isolate superoxide dismutase from garlic is proposed. The separation and purification procedure consists of phosphate buffer extraction, heat treatment and a two-stage ultrafiltration process. The enzyme was purified 139-fold with a specific activity of 2867 U/mg protein and a yield of 91%. The native molecular mass of superoxide dismutase estimated by fast protein liquid chromatography on a Superose 6 column was 28 kDa. Sodium dodecyl sulfate-polya...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/j.fbp.2010.07.003
-
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- Journal:
- FOOD AND BIOPRODUCTS PROCESSING
- Volume:
- 89
- Issue:
- C4
- Pages:
- 294-299
- Publication date:
- 2011-10-05
- DOI:
- ISSN:
-
0960-3085
- URN:
-
uuid:2df86488-1feb-419c-858f-94d6fe2d557f
- Source identifiers:
-
199247
- Local pid:
- pubs:199247
- Language:
- English
- Keywords:
- Copyright date:
- 2011
Journal article
Purification and characterization of superoxide dismutase from garlic
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