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A photochemically induced dynamic nuclear polarization study of denatured states of lysozyme.

Abstract:

Photochemically induced dynamic nuclear polarization (photo-CIDNP) techniques have been used to examine denatured states of lysozyme produced under a variety of conditions. 1H CIDNP difference spectra of lysozyme denatured thermally, by the addition of 10 M urea, or by the complete reduction of its four disulfide bonds were found to differ substantially not only from the spectrum of the native protein but also from that expected for a completely unstructured polypeptide chain. Specifically, d...

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Publication status:
Published

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Publisher copy:
10.1021/bi00216a015

Authors


Broadhurst, RW More by this author
Dobson, CM More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Radford, SE More by this author
Journal:
Biochemistry
Volume:
30
Issue:
2
Pages:
405-412
Publication date:
1991-01-05
DOI:
EISSN:
1520-4995
ISSN:
0006-2960
URN:
uuid:2dafc341-e0c8-4b56-872b-7aab5aa4e621
Source identifiers:
34954
Local pid:
pubs:34954

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