Journal article
Mass spectrometry guided structural biology.
- Abstract:
- With the convergence of breakthroughs in structural biology, specifically breaking the resolution barriers in cryo-electron microscopy and with continuing developments in crystallography, novel interfaces with other biophysical methods are emerging. Here we consider how mass spectrometry can inform these techniques by providing unambiguous definition of subunit stoichiometry. Moreover recent developments that increase mass spectral resolution enable molecular details to be ascribed to unassigned density within high-resolution maps of membrane and soluble protein complexes. Importantly we also show how developments in mass spectrometry can define optimal solution conditions to guide downstream structure determination, particularly of challenging biomolecules that refuse to crystallise.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 2.1MB, Terms of use)
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- Publisher copy:
- 10.1016/j.sbi.2016.09.008
Authors
- Publisher:
- Elsevier
- Journal:
- Current Opinion in Structural Biology More from this journal
- Volume:
- 40
- Pages:
- 136-144
- Publication date:
- 2016-10-01
- Acceptance date:
- 2016-09-07
- DOI:
- ISSN:
-
0959-440X and 1879-033X
Terms of use
- Copyright holder:
- Allison et al
- Copyright date:
- 2016
- Notes:
- © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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