CD45 ectodomain controls interaction with GEMs and Lck activity for optimal TCR signaling.

Journal article

The transmembrane phosphatase CD45 regulates both Lck activity and T cell receptor (TCR) signaling. Here we have tested whether the large ectodomain of CD45 has a role in this regulation. A CD45 chimera containing the large ectodomain of CD43 efficiently rescues TCR signaling in CD45-null T cells, whereas CD45 chimeras containing small ectodomains from other phosphatases do not. Both basal Lck activity in unstimulated cells and the TCR-induced increase in tyrosine phosphorylation of the TCR z...

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Authors: Irles, C; Symons, A; Michel, F; Bakker, T; Van Der Merwe, P

• Publication status: Published
• Journal: Nature immunology
• Volume: 4
• Issue: 2
• Pages: 189-197
• Publication date: 2003-02-01
• DOI: 10.1038/ni877
• EISSN: 1529-2916
• ISSN: 1529-2908
• Language: English
• Keywords: Antigens, CD; Lymphocyte Specific Protein Tyrosine Kinase p56(lck); Receptors, Antigen, T-Cell; Glycosphingolipids; Transfection; Signal Transduction; T-Lymphocytes; Rats; Antigens, CD2; Recombinant Fusion Proteins; Sialoglycoproteins; Membrane Lipids; Antigens, CD43; Antigens, Thy-1; Animals; Antigens, CD45; Humans; Dimerization; Cell Line; Mice; Protein Structure, Tertiary