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Unified single-cell analysis of testis gene regulation and pathology in 5 mouse strains.

Abstract:

To fully exploit the potential of single-cell functional genomics in the study of development and disease, robust methods are needed to simplify the analysis of data across samples, time-points and individuals. Here we introduce a model-based factor analysis method, SDA, to analyse a novel 57,600-cell dataset from the testes of wild-type mice and mice with gonadal defects due to disruption of the genes Mlh3, Hormad1, Cul4a or Cnp. By jointly analysing mutant and wild-type cells we decomposed ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.7554/elife.43966

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Oxford college:
Linacre College
Role:
Author
ORCID:
0000-0002-2007-8978
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Role:
Author
Publisher:
eLife Sciences Publications
Journal:
eLife More from this journal
Volume:
2019
Issue:
8
Article number:
e43966
Publication date:
2019-06-25
Acceptance date:
2019-06-17
DOI:
ISSN:
2050-084X
Pmid:
31237565
Language:
English
Keywords:
Pubs id:
pubs:1026672
UUID:
uuid:2be19690-51b3-4672-831d-3bf3747a8162
Local pid:
pubs:1026672
Source identifiers:
1026672
Deposit date:
2019-07-16

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