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Journal article

Unrestrained poly-ADP-ribosylation provides insights into chromatin regulation and human disease

Abstract:

ARH3/ADPRHL2 and PARG are the primary enzymes reversing ADP-ribosylation in vertebrates, yet their functions in vivo remain unclear. ARH3 is the only hydrolase able to remove serine-linked mono(ADP-ribose) (MAR) but is much less efficient than PARG against poly(ADP-ribose) (PAR) chains in vitro. Here, by using ARH3-deficient cells, we demonstrate that endogenous MARylation persists on chromatin throughout the cell cycle, including mitosis, and is surprisingly well tolerated. Convers...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.molcel.2021.04.028

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
ORCID:
0000-0002-5467-5586
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
ORCID:
0000-0002-9497-1742
More by this author
Institution:
University of Oxford
Division:
MSD
Sub department:
Pathology Dunn School
Role:
Author
Publisher:
Cell Press
Journal:
Molecular Cell More from this journal
Volume:
81
Issue:
12
Pages:
2640-2655
Publication date:
2021-05-12
Acceptance date:
2021-04-29
DOI:
EISSN:
1097-4164
ISSN:
1097-2765
Pmid:
34019811
Language:
English
Keywords:
Pubs id:
1178118
Local pid:
pubs:1178118
Deposit date:
2021-07-14

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