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In vivo nuclear-magnetic-resonance analysis of polyamine and alkaloid metabolism in transformed root cultures of Datura stramonium L.: evidence for the involvement of putrescine in phytohormone-induced de-differentiation

Abstract:

The utilisation and accumulation of 15N-labeled metabolites by a 15N-labeled transformed root culture of Datura stramonium L. was investigated by in vivo 15N-nuclear-magnetic-resonance (NMR) spectroscopy. After resuspension in spent growth medium, the pools of [15N]glutamate and [15N]glutamine were rapidly depleted and there was an increase in the 15N-NMR signals from conjugated putrescines and hyoscyamine. The signal from the conjugated putrescines passed through a maximum 2 d after the root...

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Publication status:
Published

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Publisher copy:
10.1007/s004250050313

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Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
Role:
Author
Journal:
PLANTA
Volume:
205
Issue:
2
Pages:
205-213
Publication date:
1998-06-05
DOI:
EISSN:
1432-2048
ISSN:
0032-0935
URN:
uuid:2a8151bc-9148-4648-91a6-deea7762ae4b
Source identifiers:
36586
Local pid:
pubs:36586

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