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Optimization of primary culture condition for mesenchymal stem cells derived from umbilical cord blood with factorial design.

Abstract:

Mesenchymal stem cells (MSCs) can not only support the expansion of hematopoietic stem cells in vitro, but also alleviate complications and accelerate recovery of hematopoiesis during hematopoietic stem cell transplantation. However, it proved challenging to culture MSCs from umbilical cord blood (UCB) with a success rate of 20-30%. Many cell culture parameters contribute to this outcome and hence optimization of culture conditions is critical to increase the probability of success. In this w...

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Publication status:
Published

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Publisher copy:
10.1002/btpr.68

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Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science
Role:
Author
Journal:
Biotechnology progress
Volume:
25
Issue:
2
Pages:
499-507
Publication date:
2009-01-01
DOI:
EISSN:
1520-6033
ISSN:
8756-7938
URN:
uuid:2a368cad-8b41-4c8c-9680-ad4cf1670962
Source identifiers:
62090
Local pid:
pubs:62090

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