Journal article icon

Journal article

A protein biosensor that relies on bending of single DNA molecules.

Abstract:
A "bendy" protein sensor: A DNA-based sensor that uses folded DNA (through DNA kinks) and protein-induced bending to detect DNA-binding proteins is presented. Single-molecule sensing of a transcriptional activator (catabolite activator protein, CAP, which bends its DNA site by 80°) is demonstrated in solution and on surfaces, both in buffers and in cell lysates. The method should allow detection of a wide range of DNA-bending proteins.
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1002/cphc.201100881

Authors


More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Physics
Kapanidis, AN More by this author
Journal:
Chemphyschem : a European journal of chemical physics and physical chemistry
Volume:
13
Issue:
4
Pages:
918-922
Publication date:
2012-03-05
DOI:
EISSN:
1439-7641
ISSN:
1439-4235
URN:
uuid:29c66517-1305-4188-b1e8-dfa89c4c0044
Source identifiers:
312829
Local pid:
pubs:312829

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP