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Proteasome activity profiling: a simple, robust and versatile method revealing subunit-selective inhibitors and cytoplasmic, defense-induced proteasome activities.

Abstract:

The proteasome plays essential roles in nearly all biological processes in plant defense and development, yet simple methods for displaying proteasome activities in extracts and living tissues are not available to plant science. Here, we introduce an easy and robust method to simultaneously display the activities of all three catalytic proteasome subunits in plant extracts or living plant tissues. The method is based on a membrane-permeable, small-molecule fluorescent probe that irreversibly ...

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Publication status:
Published

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Journal:
Plant journal : for cell and molecular biology More from this journal
Volume:
62
Issue:
1
Pages:
160-170
Publication date:
2010-04-01
DOI:
EISSN:
1365-313X
ISSN:
0960-7412
Language:
English
Keywords:
Pubs id:
pubs:411972
UUID:
uuid:28c9050c-dbc0-4b47-926d-b08ceccf4905
Local pid:
pubs:411972
Source identifiers:
411972
Deposit date:
2013-11-16

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