Journal article icon

Journal article

Site-directed mutagenesis of beta-lactamase I: role of Glu-166.

Abstract:

Two Glu-166 mutants of beta-lactamase I from Bacillus cereus 569/H were constructed: one with a lengthened side chain (E166Cmc, the S-carboxymethylcysteine mutant) and the other with the side chain shortened and made non-polar (E166A). Their kinetic properties were studied and compared with those of the wild-type and the E166D mutant (with a shortened side chain) previously made by Gibson, Christensen and Waley (1990) (Biochem. J. 272, 613-619). Surprisingly, with good penicillin substrates, ...

Expand abstract
Publication status:
Published

Actions


Authors


Robinson, CV More by this author
Journal:
The Biochemical journal
Volume:
299 ( Pt 3)
Issue:
3
Pages:
671-678
Publication date:
1994-05-05
EISSN:
1470-8728
ISSN:
0264-6021
URN:
uuid:289539b1-e4f9-4396-82e5-c6a80617ded8
Source identifiers:
59343
Local pid:
pubs:59343

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP