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Parameter scanning ultrafiltration: rapid optimisation of protein separation.

Abstract:

High-resolution fractionation of proteins using ultrafiltration is feasible only at highly optimised conditions. Conventional process optimisation methodology demands both time and material. Pulsed sample injection ultrafiltration has been suggested as a rapid process optimisation technique. In the present work the scope of this technique is further extended by "parameter scanning ultrafiltration," which involves continuous change of a process parameter (e.g., pH, salt concentration). The tim...

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Publication status:
Published

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Publisher copy:
10.1002/bit.10510

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Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science
Role:
Author
Journal:
Biotechnology and bioengineering
Volume:
81
Issue:
6
Pages:
673-682
Publication date:
2003-03-05
DOI:
EISSN:
1097-0290
ISSN:
0006-3592
URN:
uuid:288ea709-7bc5-4ffc-b5ce-125df60fb204
Source identifiers:
61963
Local pid:
pubs:61963

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