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An optimized protocol for metabolome analysis in yeast using direct infusion electrospray mass spectrometry.

Abstract:

A method for the global analysis of yeast intracellular metabolites, based on electrospray mass spectrometry (ES-MS), has been developed. This has involved the optimization of methods for quenching metabolism in Saccharomyces cerevisiae and extracting the metabolites for analysis by positive-ion electrospray mass spectrometry. The influence of cultivation conditions, sampling, quenching and extraction conditions, concentration step, and storage have all been studied and adapted to allow direc...

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Publication status:
Published

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Authors


Castrillo, JI More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Gaskell, SJ More by this author
Oliver, SG More by this author
Journal:
Phytochemistry
Volume:
62
Issue:
6
Pages:
929-937
Publication date:
2003-03-05
DOI:
EISSN:
1873-3700
ISSN:
0031-9422
URN:
uuid:287d406e-7b13-4736-a780-bfdeb229ae09
Source identifiers:
384381
Local pid:
pubs:384381

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