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Differentiating embryonic stem cells: GAPDH, but neither HPRT nor beta-tubulin is suitable as an internal standard for measuring RNA levels.

Abstract:

Embryonic stem (ES) cells are pluripotent cell lines that possess virtually unlimited self-renewal and differentiation capacity. Such characteristics make them potentially an invaluable cell source for diverse tissue-engineering applications. In vitro ES cell differentiation occurs spontaneously in three-dimensional structures termed "embryoid bodies" that mimic postimplantation embryonic tissue. HPRT, beta-tubulin, and GAPDH are commonly used as internal RNA standards in ES cell-derived gene...

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Publication status:
Published

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Publisher copy:
10.1089/107632702760240472

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Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
Journal:
Tissue engineering
Volume:
8
Issue:
4
Pages:
551-559
Publication date:
2002-08-05
DOI:
EISSN:
1557-8690
ISSN:
1076-3279
URN:
uuid:282b3165-08dd-4757-aa5a-aabf029d6560
Source identifiers:
227052
Local pid:
pubs:227052

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