Fast molecular tracking maps nanoscale dynamics of plasma membrane lipids.

Journal article

We describe an optical method capable of tracking a single fluorescent molecule with a flexible choice of high spatial accuracy (approximately 10-20 nm standard deviation or approximately 20-40 nm full-width-at-half-maximum) and temporal resolution (< 1 ms). The fluorescence signal during individual passages of fluorescent molecules through a spot of excitation light allows the sequential localization and thus spatio-temporal tracking of the molecule if its fluorescence is collected on at ...

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Authors: Sahl, S; Leutenegger, M; Hilbert, M; Hell, S; Eggeling, C

• Publication status: Published
• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 107
• Issue: 15
• Pages: 6829-6834
• Publication date: 2010-04-01
• DOI: 10.1073/pnas.0912894107
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Diffusion; Potoroidae; Reproducibility of Results; Cell Membrane; Animals; Time Factors; Microscopy, Confocal; Signal Processing, Computer-Assisted; Probability; Kidney; Membrane Lipids; Membrane Microdomains