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Agromonas: a rapid disease assay for Pseudomonas syringae growth in agroinfiltrated leaves

Abstract:

The lengthy process to generate transformed plants is a limitation in current research on the interactions of the model plant pathogen Pseudomonas syringae with plant hosts. Here we present an easy method called agromonas, where we quantify P. syringae growth in agroinfiltrated leaves of Nicotiana benthamiana using a cocktail of antibiotics to select P. syringae on plates. As a proof of concept, we demonstrate that...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1111/tpj.15056

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Institution:
University of Oxford
Division:
MPLS
Department:
Biology
Role:
Author
ORCID:
0000-0003-4756-1203
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Role:
Author
ORCID:
0000-0003-4408-8262
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Role:
Author
ORCID:
0000-0003-4677-0185
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Role:
Author
ORCID:
0000-0002-9007-1333
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Biology
Role:
Author
ORCID:
0000-0003-3882-4438
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Name:
Biotechnology and Biological Sciences Research Council
Grant:
BB/R017913/1
Publisher:
Wiley
Journal:
Plant Journal More from this journal
Volume:
105
Issue:
3
Pages:
831-840
Publication date:
2021-01-12
Acceptance date:
2020-10-21
DOI:
EISSN:
1365-313X
ISSN:
0960-7412
Pmid:
33124734

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