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Highly efficient depletion strategy for the two most abundant erythrocyte soluble proteins improves proteome coverage dramatically.

Abstract:
In-depth human erythrocyte proteome studies are severely hampered by the presence of hemoglobin and carbonic anhydrase-1, which account for more than 98% of the total erythrocyte soluble protein content. We developed a specific depletion approach that resulted in a drastic increase in the number of identified proteins. This depletion technique is valuable for proteome studies of human erythrocyte disorders with unknown etiology and of tissue samples that contain blood.
Publication status:
Published

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Publisher copy:
10.1021/pr8001029

Authors


Ringrose, JH More by this author
van Solinge, WW More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
O'Flaherty, MC More by this author
van Wijk, R More by this author
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Journal:
Journal of proteome research
Volume:
7
Issue:
7
Pages:
3060-3063
Publication date:
2008-07-05
DOI:
EISSN:
1535-3907
ISSN:
1535-3893
URN:
uuid:27250514-70cc-42af-b145-24b1d3d02594
Source identifiers:
384597
Local pid:
pubs:384597

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