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Adaptive multiphoton and harmonic generation microscopy for developmental biology

Abstract:

Specimen-induced aberrations often affect microscopes, particularly when high numerical aperture lenses are used to image deep into biological specimens. These aberrations cause a reduction in resolution and focal intensity. This is particularly problematic in multiphoton microscopy, where the non-linear nature of the signal generation process means that the signal level is strongly affected by changes in the focal spot intensity. We have applied the techniques of adaptive optics to correct a...

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Publication status:
Published

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Publisher copy:
10.1117/12.843312

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Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
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Journal:
MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES X
Volume:
7569
Publication date:
2010-01-01
DOI:
ISSN:
0277-786X
Source identifiers:
106983
Language:
English
Keywords:
Pubs id:
pubs:106983
UUID:
uuid:26dbaad3-e857-48c9-b1a9-711f73aa03cb
Local pid:
pubs:106983
Deposit date:
2012-12-19

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