The formation of single-stranded breaks in DNA following UV irradiation is assessed in uvrC34 mutants. By altering the SOS DNA-repair system, either by additional mutations or by using drugs affecting transcription or translation, it is shown that such single-stranded breaks require one or more DNA-damage-inducible functions. A UV-sensitive strain is characterized as carrying a Tn10 insertion into the uvrC gene. The absence of post-irradiation incision in this strain demonstrates that uvrC fu...Expand abstract
- Journal of general microbiology
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A transposon insertion in the Escherichia coli uvrC gene; UvrC protein is absolutely required for the incision step in excision repair.
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