Journal article
Multiphoton time-domain fluorescence lifetime imaging microscopy: practical application to protein-protein interactions using global analysis
- Abstract:
-
Förster resonance energy transfer (FRET) detected via fluorescence lifetime imaging microscopy (FLIM) and global analysis provide a way in which protein-protein interactions may be spatially localized and quantified within biological cells. The FRET efficiency and proportion of interacting molecules have been determined using bi-exponential fitting to time-domain FLIM data from a multiphoton time-correlated single-photon counting microscope system. The analysis has been made more robust to no...
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- Publication status:
- Published
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- Publisher copy:
- 10.1098/rsif.2008.0451.focus
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Bibliographic Details
- Journal:
- JOURNAL OF THE ROYAL SOCIETY INTERFACE
- Volume:
- 6
- Issue:
- SUPPL. 1
- Pages:
- S93-S105
- Publication date:
- 2009-02-06
- DOI:
- EISSN:
-
1742-5662
- ISSN:
-
1742-5689
- Source identifiers:
-
132100
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:132100
- UUID:
-
uuid:25f92c85-4d22-47c8-90b7-d41c3f86f8d5
- Local pid:
- pubs:132100
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 2009
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