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Multiphoton time-domain fluorescence lifetime imaging microscopy: practical application to protein-protein interactions using global analysis

Abstract:

Förster resonance energy transfer (FRET) detected via fluorescence lifetime imaging microscopy (FLIM) and global analysis provide a way in which protein-protein interactions may be spatially localized and quantified within biological cells. The FRET efficiency and proportion of interacting molecules have been determined using bi-exponential fitting to time-domain FLIM data from a multiphoton time-correlated single-photon counting microscope system. The analysis has been made more robust to no...

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Publication status:
Published

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Journal:
JOURNAL OF THE ROYAL SOCIETY INTERFACE
Volume:
6
Issue:
SUPPL. 1
Pages:
S93-S105
Publication date:
2009-02-06
DOI:
EISSN:
1742-5662
ISSN:
1742-5689
URN:
uuid:25f92c85-4d22-47c8-90b7-d41c3f86f8d5
Source identifiers:
132100
Local pid:
pubs:132100

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