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Journal article

Objective-type dark-field illumination for scattering from microbeads.

Abstract:

We introduce a method for detecting and tracking small particles in a solution near a surface. The method is based on blocking the backreflected illumination beam in an objective-type total internal reflection microscope, leaving unhindered the light scattered by the particles and resulting in dark-field illumination. Using this method, we tracked the motion of 60-nm polystyrene beads with a signal-to-noise ratio of 6 and detected 20-nm gold particles with a signal-to-noise ratio of 5. We ill...

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Publication status:
Published

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Publisher copy:
10.1364/ao.40.005650

Authors


Braslavsky, I More by this author
Jaffar Ali, BM More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Genomics Consortium
Oppenheim, A More by this author
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Journal:
Applied optics
Volume:
40
Issue:
31
Pages:
5650-5657
Publication date:
2001-11-05
DOI:
EISSN:
1539-4522
ISSN:
0003-6935
URN:
uuid:25d3d9db-781f-49e3-8ece-137e10475f72
Source identifiers:
34182
Local pid:
pubs:34182
Language:
English

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