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Efficient characterisation of human cell-bioceramic interactions in vitro and in vivo by using enhanced GFP-labelled mesenchymal stem cells.

Abstract:

Human mesenchymal stem cells (hMSCs) were transfected using four retroviral pseudotypes, amphotropic murine leukemia viruses 4070 (MuLV-10A1), a modification of amphotropic pseudotype 4073 (A71G, Q74K, V139M), gibbon ape leukemia virus (GaLV), or feline endogenous virus (RD114) encoding the neomycin resistance (Neo(r)) gene and enhanced green fluorescent protein (eGFP) as genetic markers. It was observed that the MuLV4073 was the most efficient pseudotype for hMSC transfection. The proliferat...

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Publication status:
Published

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Authors


Locklin, RM More by this author
Ferguson, DJ More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science
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Journal:
Biomaterials
Volume:
26
Issue:
29
Pages:
5790-5800
Publication date:
2005-10-05
DOI:
EISSN:
1878-5905
ISSN:
0142-9612
URN:
uuid:256a1a0d-7c52-427e-9613-7da474a1a850
Source identifiers:
61889
Local pid:
pubs:61889

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