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Fluorinated amphiphiles control the insertion of α-hemolysin pores into lipid bilayers.

Abstract:

The insertion of fully folded and assembled ion channels and pores into planar lipid bilayers for electrical recording has been facilitated by the use of conventional detergents at a final concentration below the critical micelle concentration (CMC). After the desired number of channels or pores (often one) has been incorporated into a bilayer, it is important to prevent further insertion events, which is often done by awkward techniques such as perfusion. Here, we show that the addition of s...

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Publication status:
Published

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Publisher copy:
10.1021/bi1012386

Authors


Raychaudhuri, P More by this author
Mikhailova, E More by this author
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Journal:
Biochemistry
Volume:
50
Issue:
10
Pages:
1599-1606
Publication date:
2011-03-05
DOI:
EISSN:
1520-4995
ISSN:
0006-2960
URN:
uuid:2548ffa6-e1c7-4f1f-8708-f6ec5d7afbb9
Source identifiers:
115740
Local pid:
pubs:115740

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