Regulated exocytosis of GABA-containing synaptic-like microvesicles in pancreatic beta-cells.

Journal article

We have explored whether gamma-aminobutyric acid (GABA) is released by regulated exocytosis of GABA-containing synaptic-like microvesicles (SLMVs) in insulin-releasing rat pancreatic beta-cells. To this end, beta-cells were engineered to express GABA(A)-receptor Cl(-)-channels at high density using adenoviral infection. Electron microscopy indicated that the average diameter of the SLMVs is 90 nm, that every beta-cell contains approximately 3,500 such vesicles, and that insulin-containing lar...

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Authors: Braun, M; Wendt, A; Birnir, B; Broman, J; Eliasson, L

• Publication status: Published
• Journal: Journal of general physiology
• Volume: 123
• Issue: 3
• Pages: 191-204
• Publication date: 2004-03-01
• DOI: 10.1085/jgp.200308966
• EISSN: 1540-7748
• ISSN: 0022-1295
• Language: English
• Keywords: Islets of Langerhans; Calcium Signaling; Humans; Rats, Wistar; GABA-A Receptor Agonists; Synaptic Vesicles; Dose-Response Relationship, Drug; gamma-Aminobutyric Acid; Rats; Receptors, GABA-A; Exocytosis; Animals; Rats, Sprague-Dawley