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GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System.

Abstract:

A key step in the production of recombinant membrane proteins for structural studies is the optimization of protein yield and quality. One commonly used approach is to fuse the protein to green fluorescent protein (GFP), enabling expression to be tracked without the need to purify the protein. Combining fusion to green fluorescent protein with the baculovirus expression system provides a useful platform for both screening and production of eukaryotic membrane proteins. In this chapter we desc...

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Publisher:
Humana Press Inc.
Journal:
Methods in molecular biology (Clifton, N.J.) More from this journal
Volume:
1261
Pages:
197-209
Publication date:
2015-01-01
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
Language:
English
Keywords:
Pubs id:
pubs:500769
UUID:
uuid:24db906b-f7a7-4c7a-9796-1a5acbcbdd7e
Local pid:
pubs:500769
Source identifiers:
500769
Deposit date:
2015-01-12

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