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Identification of Single Molecules in Aqueous Solution by Time-Resolved Fluorescence Anisotropy

Abstract:

Using a confocal epi-illuminated microscope with a polarizing beam splitter and dual-channel detection of single-molecule fluorescence induced by pulsed laser excitation, a new application of the three-dimensional, real-time spectroscopic technique BIFL (burst integrated fluorescence lifetime) is introduced. BIFL allows simultaneous registration of fluorescence intensity, lifetime, and anisotropy. It is shown to be well-suited to identify the freely diffusing fluorescent molecule Rhodamine 12...

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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
Journal:
Journal of Physical Chemistry A More from this journal
Volume:
103
Issue:
3
Publication date:
1999-01-01
ISSN:
1089-5639
Language:
English
Pubs id:
pubs:222216
UUID:
uuid:2454815d-e177-4739-878d-aed29224de7b
Local pid:
pubs:222216
Source identifiers:
222216
Deposit date:
2013-11-16

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