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A functional human Poly(A) site requires only a potent DSE and an A-rich upstream sequence

Abstract:

We have analysed the sequences required for cleavage and polyadenylation in the intronless melanocortin 4 receptor (MC4R) pre-mRNA. Unlike other intronless genes, 3′end processing of the MC4R primary transcript is independent of any auxiliary sequence elements and only requires the core poly(A) sequences. Mutation of the AUUAAA hexamer had little effect on MC4R 3′end processing but small changes in the short DSE severely reduced cleavage efficiency. The MC4R poly(A) site requires only the DSE...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/emboj.2010.42

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Research group:
Laboratory of Genes and Development
Role:
Author
More by this author
Institution:
UMDNJ - New Jersey Medical School, Newark, NJ, USA
Department:
Department of Biochemistry and Molecular Biology
Role:
Author
More by this author
Institution:
UMDNJ - New Jersey Medical School, Newark, NJ, USA
Department:
Department of Biochemistry and Molecular Biology
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author

Contributors

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Name:
Wellcome Trust
Funding agency for:
Furger, A
Grant:
081083/Z/06/Z
More from this funder
Name:
NIH, United States
Funding agency for:
Li, W
Tian, B
More from this funder
Name:
Portuguese FCT
Funding agency for:
Nunes, N
Publisher:
Nature Publishing Group
Journal:
EMBO Journal More from this journal
Volume:
29
Issue:
9
Pages:
1523-1536
Publication date:
2010-05-01
DOI:
EISSN:
1460-2075
ISSN:
0261-4189
Language:
English
Subjects:
UUID:
uuid:244b3763-ef28-4226-acab-5f2377f1cf4f
Local pid:
ora:3779
Deposit date:
2010-05-14

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