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The effect of protein complexation on the mechanical stability of Im9.

Abstract:

Force mode microscopy can be used to examine the effect of mechanical manipulation on the noncovalent interactions that stabilize proteins and their complexes. Here we describe the effect of complexation by the high affinity protein ligand E9 on the mechanical resistance of the simple four-helical protein, Im9. When concatenated into a construct of alternating I27 domains, Im9 unfolded below the thermal noise limit of the instrument ( approximately 20 pN). Complexation of E9 had little effect...

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Publisher copy:
10.1529/biophysj.106.102475

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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
Role:
Author
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Journal:
Biophysical journal
Volume:
92
Issue:
9
Pages:
L79-L81
Publication date:
2007-05-05
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
URN:
uuid:24300d06-1cbb-425f-b818-42b982ac1cf0
Source identifiers:
310223
Local pid:
pubs:310223

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