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Quantifying cell death induced by doxorubicin, hyperthermia or HIFU ablation with flow cytometry

Abstract:

Triggered release and targeted drug delivery of potent anti-cancer agents using hyperthermia-mediated focused-ultrasound (FUS) is gaining momentum in the clinical setting. In early phase studies, tissue biopsy samples may be harvested to assess drug delivery efficacy and demonstrate lack of instantaneous cell death due to FUS exposure. We present an optimised tissue cell recovery method and a cell viability assay, compatible with intra-cellular doxorubicin. Flow cytometry was used to determin...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41598-021-83845-2

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Surgical Sciences
Role:
Author
ORCID:
0000-0002-8432-5771
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Name:
Engineering & Physical Sciences Research Council
Grant:
EP/L024012/1
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Name:
Oxford Centre for Drug Delivery Devices
Publisher:
Springer Nature
Journal:
Scientific Reports More from this journal
Volume:
11
Issue:
1
Article number:
4404
Publication date:
2021-02-23
Acceptance date:
2021-02-04
DOI:
EISSN:
2045-2322
Language:
English
Keywords:
Pubs id:
1163899
Local pid:
pubs:1163899
Deposit date:
2021-04-03

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