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Journal article

Inefficient cationic lipid-mediated siRNA and antisense oligonucleotide transfer to airway epithelial cells in vivo.

Abstract:

BACKGROUND: The cationic lipid Genzyme lipid (GL) 67 is the current "gold-standard" for in vivo lung gene transfer. Here, we assessed, if GL67 mediated uptake of siRNAs and asODNs into airway epithelium in vivo. METHODS: Anti-lacZ and ENaC (epithelial sodium channel) siRNA and asODN were complexed to GL67 and administered to the mouse airway epithelium in vivo Transfection efficiency and efficacy were assessed using real-time RT-PCR as well as through protein expression and functional studie...

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Publication status:
Published

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Publisher copy:
10.1186/1465-9921-7-26

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Journal:
Respiratory research
Volume:
7
Pages:
26
Publication date:
2006-01-01
DOI:
EISSN:
1465-993X
ISSN:
1465-9921
Source identifiers:
246460
Language:
English
Keywords:
Pubs id:
pubs:246460
UUID:
uuid:241b4805-ae85-4797-b0e4-8ecaf5edf640
Local pid:
pubs:246460
Deposit date:
2012-12-19

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