Inefficient cationic lipid-mediated siRNA and antisense oligonucleotide transfer to airway epithelial cells in vivo.

Journal article

BACKGROUND: The cationic lipid Genzyme lipid (GL) 67 is the current "gold-standard" for in vivo lung gene transfer. Here, we assessed, if GL67 mediated uptake of siRNAs and asODNs into airway epithelium in vivo. METHODS: Anti-lacZ and ENaC (epithelial sodium channel) siRNA and asODN were complexed to GL67 and administered to the mouse airway epithelium in vivo Transfection efficiency and efficacy were assessed using real-time RT-PCR as well as through protein expression and functional studie...

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Authors: Griesenbach, U; Kitson, C; Escudero Garcia, S; Farley, R; Singh, C

• Publication status: Published
• Journal: Respiratory research
• Volume: 7
• Pages: 26
• Publication date: 2006-01-01
• DOI: 10.1186/1465-9921-7-26
• EISSN: 1465-993X
• ISSN: 1465-9921
• Language: English
• Keywords: Cells, Cultured; Oligonucleotides, Antisense; Humans; Transfection; Mice; Cystic Fibrosis; Gene Silencing; Animals; NIH 3T3 Cells; Epithelial Cells; Lipids; Gene Targeting; RNA, Small Interfering; Respiratory Mucosa