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Giemsa-stained wet mount based method for reticulocyte quantification: a viable alternative in resource limited or malaria endemic settings

Abstract:

The quantity of circulating reticulocytes is an important indicator of erythropoietic activity in response to a wide range of haematological pathologies. While most modern laboratories use flow cytometry to quantify reticulocytes, most field laboratories still rely on ‘subvital’ staining. The specialist ‘subvital’ stains, New Methylene Blue (NMB) and Brilliant Crésyl Blue are often difficult to procure, toxic, and show inconsistencies between batches. Here we demonstrate the utility of Giemsa...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1371/journal.pone.0060303

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Publisher:
Public Library of Science
Journal:
PLoS ONE More from this journal
Volume:
8
Issue:
4
Pages:
ARTN e60303
Publication date:
2013-04-02
Acceptance date:
2013-02-25
DOI:
EISSN:
1932-6203
ISSN:
1932-6203
Language:
English
Keywords:
UUID:
uuid:24113c28-fd03-49ef-b880-2ae91c296952
Local pid:
pubs:394886
Source identifiers:
394886
Deposit date:
2014-02-08

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