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A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples.

Abstract:

BACKGROUND:
Sequencing technology advancements opened new opportunities to use transcriptomics for studying malaria pathology and epidemiology. Even though in recent years the study of whole parasite transcriptome proved to be essential in understanding parasite biology there is no compiled up-to-date reference protocol for the efficient generation of transcriptome data from growing number of samples. Here, a comprehensive methodology on how to preserve, extract, amplify, and sequence full...

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Publication status:
Published
Peer review status:
Peer reviewed

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Files:
Publisher copy:
10.1186/s12936-020-03436-w

Authors


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Role:
Author
ORCID:
0000-0002-1505-5608
More by this author
Role:
Author
ORCID:
0000-0003-3461-4767
Singapore National Medical Research Council More from this funder
Publisher:
BioMed Central Publisher's website
Journal:
Malaria Journal Journal website
Volume:
19
Issue:
1
Article number:
363
Publication date:
2020-10-09
Acceptance date:
2020-10-01
DOI:
ISSN:
1475-2875
Pmid:
33036628
Language:
English
Keywords:
Pubs id:
1137856
Local pid:
pubs:1137856
Deposit date:
2020-11-05

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