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Rate of incorporation of radiolabelled nucleosides does not necessarily reflect the metabolic state of cells in culture: effects of latent mycoplasma contamination.

Abstract:

In response to cell-free conditioned medium derived from the human bladder carcinoma line T24 (T24 SN), we found greatly reduced incorporation of tritiated thymidine and uridine ([3H]TdR, [3H]UR) by the human carcinoma lines UCHNCu (small-cell lung carcinoma) and LS174T (colon carcinoma). The effect was not due to an excess of nucleosides or cytokines known to be present in T24 SN. Cell-cycle distribution, increase in cell numbers, and de novo nucleoside synthesis in the indicator cells were ...

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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
NDM Experimental Medicine
Role:
Author
Journal:
Immunology
Volume:
63
Issue:
1
Pages:
125-131
Publication date:
1988-01-01
EISSN:
1365-2567
ISSN:
0019-2805
Source identifiers:
35319
Language:
English
Keywords:
Pubs id:
pubs:35319
UUID:
uuid:238414e4-db3c-47ee-980e-cf8ac669742d
Local pid:
pubs:35319
Deposit date:
2012-12-20

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