Rate of incorporation of radiolabelled nucleosides does not necessarily reflect the metabolic state of cells in culture: effects of latent mycoplasma contamination.

Journal article

In response to cell-free conditioned medium derived from the human bladder carcinoma line T24 (T24 SN), we found greatly reduced incorporation of tritiated thymidine and uridine ([3H]TdR, [3H]UR) by the human carcinoma lines UCHNCu (small-cell lung carcinoma) and LS174T (colon carcinoma). The effect was not due to an excess of nucleosides or cytokines known to be present in T24 SN. Cell-cycle distribution, increase in cell numbers, and de novo nucleoside synthesis in the indicator cells were ...

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Authors: Merkenschlager, M; Kardamakis, D; Rawle, F; Spurr, N; Beverley, P

• Journal: Immunology
• Volume: 63
• Issue: 1
• Pages: 125-131
• Publication date: 1988-01-01
• EISSN: 1365-2567
• ISSN: 0019-2805
• Language: English
• Keywords: Colonic Neoplasms; Cell Line; Tritium; Mycoplasma Infections; Urinary Bladder Neoplasms; Uridine; Lung Neoplasms; Tumor Cells, Cultured; Thymidine; Humans; Pyrimidine Nucleosides