Journal article icon

Journal article

DNA extraction from primary liquid blood cultures for bloodstream infection diagnosis using whole genome sequencing

Abstract:
Purpose Speed of bloodstream infection diagnosis is vital to reduce morbidity and mortality. Whole genome sequencing (WGS) performed directly from liquid blood culture could provide single-assay species and antibiotic susceptibility prediction; however, high inhibitor and human cell/DNA concentrations limit pathogen recovery. We develop a method for the preparation of bacterial DNA for WGS-based diagnostics direct from liquid blood culture. Methodology We eva... Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed

Actions


Access Document


Files:
Publisher copy:
10.1099/jmm.0.000664

Authors


More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM; NDM Experimental Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM; NDM Experimental Medicine
Role:
Author
Expand authors...
More from this funder
Funding agency for:
Iqbal, Z
Grant:
Sir Henry Dale Fellowship (grant 102541/Z/13/Z
More from this funder
Funding agency for:
Iqbal, Z
Grant:
Sir Henry Dale Fellowship (grant 102541/Z/13/Z
More from this funder
Grant:
HealthProtectionResearchUnit(NIHRHPRU)in HealthcareAssociatedInfections
AntimicrobialResistance
Publisher:
Society for General Microbiology Publisher's website
Journal:
Journal of Medical Microbiology Journal website
Volume:
67
Issue:
3
Pages:
347-357
Publication date:
2018-01-10
Acceptance date:
2017-12-08
DOI:
EISSN:
1473-5644
ISSN:
0022-2615
Keywords:
Pubs id:
pubs:811341
UUID:
uuid:23626050-797a-41e7-bb7a-88337786c11e
Local pid:
pubs:811341
Deposit date:
2017-12-15

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP