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Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence.

Abstract:

The specificity (S) subunit of the restriction enzyme EcoKI imparts specificity for the sequence AAC(N6)GTGC. Substitution of thymine with bromodeoxyuridine in a 25 bp DNA duplex containing this sequence stimulated UV light-induced covalent crosslinking to the S subunit. Crosslinking occurred only at the residue complementary to the first adenine in the AAC sequence, demonstrating a close contact between the major groove at this sequence and the S subunit. Peptide sequencing of a proteolytica...

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Publication status:
Published

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Publisher copy:
10.1093/nar/23.7.1177

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
Journal:
Nucleic acids research More from this journal
Volume:
23
Issue:
7
Pages:
1177-1183
Publication date:
1995-04-01
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Language:
English
Keywords:
Pubs id:
pubs:400082
UUID:
uuid:2348b91d-9d4c-4fe0-811c-c7752b8d7b6b
Local pid:
pubs:400082
Source identifiers:
400082
Deposit date:
2013-11-16

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