Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence.

Journal article

The specificity (S) subunit of the restriction enzyme EcoKI imparts specificity for the sequence AAC(N6)GTGC. Substitution of thymine with bromodeoxyuridine in a 25 bp DNA duplex containing this sequence stimulated UV light-induced covalent crosslinking to the S subunit. Crosslinking occurred only at the residue complementary to the first adenine in the AAC sequence, demonstrating a close contact between the major groove at this sequence and the S subunit. Peptide sequencing of a proteolytica...

Expand abstract

Authors: Chen, A; Powell, L; Dryden, D; Murray, N; Brown, T

• Publication status: Published
• Journal: Nucleic acids research
• Volume: 23
• Issue: 7
• Pages: 1177-1183
• Publication date: 1995-04-01
• DOI: 10.1093/nar/23.7.1177
• EISSN: 1362-4962
• ISSN: 0305-1048
• Language: English
• Keywords: DNA Restriction Enzymes; Tyrosine; Binding Sites; Cross-Linking Reagents; Substrate Specificity; Base Sequence; Bromodeoxyuridine; Trypsin; Molecular Sequence Data; DNA; Sequence Homology, Amino Acid; Ultraviolet Rays; Amino Acid Sequence