Journal article
Reverse transcription through a bulky triazole linkage in RNA: implications for RNA sequencing
- Abstract:
- A triazole linkage is formed in RNA by untemplated strain-promoted or CuAAC chemical ligation of 3'-azide and 5'-cyclooctyne oligonucleotides under denaturing conditions. Reverse transcriptase reads through these artificial linkages with omission of one nucleotide. These surprising results have implications for RNA isolation, amplification, sequencing and a variety of biological applications.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Bibliographic Details
- Publisher:
- Royal Society of Chemistry Publisher's website
- Journal:
- Chemical Communications Journal website
- Volume:
- 50
- Issue:
- 57
- Pages:
- 7597-7600
- Publication date:
- 2014-05-29
- Acceptance date:
- 2014-05-22
- DOI:
- EISSN:
-
1364-548X
- ISSN:
-
1359-7345
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:469644
- UUID:
-
uuid:230ffffc-f2b0-4463-a0c4-ff72217e0409
- Local pid:
- pubs:469644
- Source identifiers:
-
469644
- Deposit date:
- 2016-03-01
Terms of use
- Copyright holder:
- The Royal Society of Chemistry
- Copyright date:
- 2014
- Notes:
- © The Royal Society of Chemistry 2014. This is the accepted manuscript version of the article. The final version of the record is available from The Royal Society of Chemistry at: https://doi.org/10.1039/c4cc03027c
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