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Reverse transcription through a bulky triazole linkage in RNA: implications for RNA sequencing

Abstract:
A triazole linkage is formed in RNA by untemplated strain-promoted or CuAAC chemical ligation of 3'-azide and 5'-cyclooctyne oligonucleotides under denaturing conditions. Reverse transcriptase reads through these artificial linkages with omission of one nucleotide. These surprising results have implications for RNA isolation, amplification, sequencing and a variety of biological applications.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1039/c4cc03027c

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
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Name:
Biotechnology and Biological Sciences Research Council
Grant:
BB/J001694/1
Publisher:
Royal Society of Chemistry
Journal:
Chemical Communications More from this journal
Volume:
50
Issue:
57
Pages:
7597-7600
Publication date:
2014-05-29
Acceptance date:
2014-05-22
DOI:
EISSN:
1364-548X
ISSN:
1359-7345
Language:
English
Keywords:
Pubs id:
pubs:469644
UUID:
uuid:230ffffc-f2b0-4463-a0c4-ff72217e0409
Local pid:
pubs:469644
Source identifiers:
469644
Deposit date:
2016-03-01

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