High GATA-2 expression inhibits human hematopoietic stem and progenitor cell function by effects on cell cycle.

Journal article

Evidence suggests the transcription factor GATA-2 is a critical regulator of murine hematopoietic stem cells. Here, we explore the relation between GATA-2 and cell proliferation and show that inducing GATA-2 increases quiescence (G(0) residency) of murine and human hematopoietic cells. In human cord blood, quiescent fractions (CD34(+)CD38(-)Hoechst(lo)Pyronin Y(lo)) express more GATA-2 than cycling counterparts. Enforcing GATA-2 expression increased quiescence of cord blood cells, reducing pr...

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Authors: Tipping, A; Pina, C; Castor, A; Hong, D; Rodrigues, N

• Publication status: Published
• Journal: Blood
• Volume: 113
• Issue: 12
• Pages: 2661-2672
• Publication date: 2009-03-01
• DOI: 10.1182/blood-2008-06-161117
• EISSN: 1528-0020
• ISSN: 0006-4971
• Language: English
• Keywords: Hematopoietic Stem Cells; Cell Cycle; Genes, Synthetic; Tamoxifen; Receptors, Estrogen; G0 Phase; Gene Expression Regulation; Apoptosis; Genes, cdc; Recombinant Fusion Proteins; Fetal Blood; Mice, Inbred NOD; Interleukin-3; GATA2 Transcription Factor; Animals; Mice, SCID; Estradiol; Humans; Cells, Cultured; Cord Blood Stem Cell Transplantation; Transcription, Genetic; Mice