Journal article icon

Journal article

RNA polymerase II activity is located on the surface of protein-rich transcription factories.

Abstract:

We used electron spectroscopic imaging to map nucleoplasmic transcription sites in human cells at unprecedented resolution. HeLa cells were permeabilised, nascent transcripts were extended in BrUTP by approximately 40 nucleotides and the resulting BrRNA immunolabelled with gold particles before structures were viewed. Nascent RNA is almost invariably associated with polymorphic and nitrogen-rich (but phosphorus-poor) structures with a diameter of approximately 87 nm and mass of 10 MDa (calcul...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1242/jcs.027250

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Journal of cell science
Volume:
121
Issue:
Pt 12
Pages:
1999-2007
Publication date:
2008-06-01
DOI:
EISSN:
1477-9137
ISSN:
0021-9533
Source identifiers:
18939
Language:
English
Keywords:
Pubs id:
pubs:18939
UUID:
uuid:22e92162-7a27-4bc2-9819-a8cf7ff665fd
Local pid:
pubs:18939
Deposit date:
2012-12-19

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP