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RNA polymerase II activity is located on the surface of protein-rich transcription factories.

Abstract:

We used electron spectroscopic imaging to map nucleoplasmic transcription sites in human cells at unprecedented resolution. HeLa cells were permeabilised, nascent transcripts were extended in BrUTP by approximately 40 nucleotides and the resulting BrRNA immunolabelled with gold particles before structures were viewed. Nascent RNA is almost invariably associated with polymorphic and nitrogen-rich (but phosphorus-poor) structures with a diameter of approximately 87 nm and mass of 10 MDa (calcul...

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Publication status:
Published

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Publisher copy:
10.1242/jcs.027250

Authors


Carter, DR More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Journal:
Journal of cell science
Volume:
121
Issue:
Pt 12
Pages:
1999-2007
Publication date:
2008-06-05
DOI:
EISSN:
1477-9137
ISSN:
0021-9533
URN:
uuid:22e92162-7a27-4bc2-9819-a8cf7ff665fd
Source identifiers:
18939
Local pid:
pubs:18939

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